Nitric oxide attenuates the expression of natriuretic peptide receptor C and associated adenylyl cyclase signaling in aortic vascular smooth muscle cells: role of MAPK.

نویسندگان

  • Maria Arejian
  • Yuan Li
  • Madhu B Anand-Srivastava
چکیده

We have earlier shown that the treatment of A10 vascular smooth muscle cells with S-nitroso-N-acetyl-penicillamine (SNAP); nitric oxide donor (NO) for 24 h decreased the expression of natriuretic peptide receptor C (NPR-C) and adenylyl cyclase signaling. The present study was undertaken to examine the implication of different signaling mechanisms in a NO-induced response. The treatment of A10 vascular smooth muscle cells with SNAP decreased the expression of NPR-C and G(i)alpha proteins in a time-dependent manner. The expression of G(i)alpha proteins was decreased at 6 h, whereas the expression of NPR-C was attenuated at 2 h. The NPR-C-mediated inhibition of adenylyl cyclase was attenuated (approximately 50%) after 2 h of treatment and was completely abolished after 6 h of treatment. The decreased expression of NPR-C and NPR-C-mediated attenuation of adenylyl cyclase after 2 h of treatment was reversed to control levels by PD-98059, a MEK inhibitor. SNAP also modulated the ERK1/2 phosphorylation in a time-dependent manner; an increase was observed up to 2 h, and, thereafter, the ERK1/2 phosphorylation was decreased. On the other hand, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one and KT-5823 inhibitor of soluble guanylyl cyclase and protein kinase G, respectively, and Mn(III)tetrakis(4-benzoic acid)porphyrin, a scavenger of peroxynitrite, were unable to restore the SNAP-induced decreased expression of NPR-C protein and increased ERK1/2 phosphorylation to control levels. However, the decreased levels of phosphorylated ERK1/2 and G(i)alpha proteins were restored to control levels by 8-bromo-cAMP. These results indicate that a temporal relationship follows between a NO-induced decreased expression of NPR-C and G(i)alpha proteins. The decreased expression of NPR-C is mediated through cGMP-independent but MAPK-dependent pathway, whereas NO-induced decreased levels of cAMP may contribute to the decreased activation of MAPK and thereby decreased the expression of G(i)alpha proteins.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Plasminogen System Expression in Vascular Smooth Muscle Cells : Role of cGMP in the Regulation of the Natriuretic Factors and Nitric Oxide Suppress Plasminogen Activator Inhibitor-1

Increased expression of plasminogen activator inhibitor-1 (PAI-1) has been reported in atherosclerotic and balloon-injured vessels. Little is known regarding the factors and mechanisms that may negatively regulate PAI-1 expression. In this report, the effect of cGMP-coupled vasoactive hormones, including natriuretic factors and nitric oxide, on the regulation of PAI-1 expression in vascular smo...

متن کامل

G protein-dependent activation of smooth muscle eNOS via natriuretic peptide clearance receptor.

In gastrointestinal smooth muscle, the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) induce relaxation by interacting with VIP2/PACAP3receptors coupled via Gs to adenylyl cyclase and with distinct receptors coupled via Gi1 and/or Gi2 to a smooth muscle endothelial nitric oxide synthase (eNOS). The present study identifies the re...

متن کامل

Role of oxidative stress in high glucose-induced decreased expression of Gi proteins and adenylyl cyclase signaling in vascular smooth muscle cells

Li Y, Descorbeth M, Anand-Srivastava MB. Role of oxidative stress in high glucose-induced decreased expression of Gi proteins and adenylyl cyclase signaling in vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 294: H2845–H2854, 2008. First published April 25, 2008; doi:10.1152/ajpheart.91422.2007.—We have recently shown that aorta from streptozotocin (STZ)-induced diabetic rats and ...

متن کامل

Natriuretic factors and nitric oxide suppress plasminogen activator inhibitor-1 expression in vascular smooth muscle cells. Role of cGMP in the regulation of the plasminogen system.

Increased expression of plasminogen activator inhibitor-1 (PAI-1) has been reported in atherosclerotic and balloon-injured vessels. Little is known regarding the factors and mechanisms that may negatively regulate PAI-1 expression. In this report, the effect of cGMP-coupled vasoactive hormones, including natriuretic factors and nitric oxide, on the regulation of PAI-1 expression in vascular smo...

متن کامل

Increased sensitivity to endothelial nitric oxide/NO contributes to arterial normotension in mice with vascular smooth muscle - selective deletion of the ANP-receptor

Running title: Vascular interactions between ANP and NO Classification: Biological Sciences, Physiology Manuscript information: 21 pages, 6 figures; abstract with 245 words, 5151 words in total. Abbreviations footnote: ANP, atrial natriuretic peptide; CNP, C-type natriuretic peptide; NO, nitric oxide; GC-A, guanylyl cyclase-A; sGC, soluble guanylyl cyclase; PKG I, cGMPdependent Protein Kinase I...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • American journal of physiology. Heart and circulatory physiology

دوره 296 6  شماره 

صفحات  -

تاریخ انتشار 2009